After Real Madrid’s refusal to give Barcelona the traditional guard of honour in respect for their recent La Liga title victory, the technical staff at the club rose to the occasion and applauded the players as they entered the pitch at the Nou CampPrior to Sunday’s “El Clasico”, Zinedine Zidane had announced that Real would not be giving their arch-rivals the guard of honour, after sealing the league title last weekend, due to the fact that Barcelona had failed to do the gesture themselves when the sides met at the Santiago Bernabeu in December after his side had won the FIFA Club World Cup.Despite the protests of both Barcelona and the Spanish press, Real remained firm on their decision and carried on with their pre-match preparations as normal.In light of this, Gerard Pique asked the technical staff to do the honour in Real’s place and so they applauded the team as they entered their home ground for the first time in front of their fans as Copa del Rey and La Liga champions.The coaching staff perform a guard of honor for the team ???? ???? Força Barça! pic.twitter.com/ypHdltokGaZidane reveals Sergio Ramos injury concern for Real Madrid Andrew Smyth – September 14, 2019 Zinedine Zidane has put Sergio Ramos’ availability for Real Madrid’s trip to Sevilla next weekend in doubt after withdrawing him against Levante.— FC Barcelona ???? (@FCBarcelona) May 6, 2018“As they didn’t want to give us a guard of honour, I ask our staff to do it and we’ll do it like that,” said Pique, as reported on Sport.The match between the two La Liga giants ended 2-2.
Liverpool midfielder Georginio Wijnaldum hailed the impact Virgil van Dijk has made in the club’s defence upon his arrival in JanuaryThe Reds signed Van Dijk for a record £75m fee for a defender in the last winter transfer window from fellow Premier League side Southampton.Since then, Van Dijk has become a crucial member of the Liverpool defence with his solid displays helping the club concede just five times in 12 Premier League matches.And Wijnaldum believes a large part of the credit should go to his fellow Dutchman.“I think Virgil has made a difference in the last line because he is a good defender, a big defender,” Wijnaldum told the club website.“He is an extra man who can defend set-pieces as well.Daniel Farke, From mid-table in the Championship to the Premier League Manuel R. Medina – September 14, 2019 Norwich City manager, Daniel Farke, has taken his team from the middle of the table in the English Championship to play with the big boys in the Premier League.“He is a leader in the last line who not only helps himself with his quality but also the players next to him and so he really helps the team.“But it is not only Virgil, the team helps him also – the players who play next to him or in front of him and the goalkeeper too because we all have to communicate with each other and help each other.“It is good teamwork that makes us do things so well, but of course he has made a real difference since he came.”Van Dijk has made 16 appearances for Liverpool in all competitions this season.Jurgen Klopp’s side will travel to Watford for their next Premier League match on November 24.
WILMINGTON, MA — The following four children were baptized and joined the Parish of the Transfiguration on Sunday, August 4, 2019:Anthony Joseph RuplisValentina Elizabeth SanchezMadelyn ShalkoskiCallen James SutherlandCongratulations to these children and their families.(NOTE: List is from the latest St. Thomas/St. Dorothy church bulletin. The cover photo is from Airgoz Aerial Photography.)Like Wilmington Apple on Facebook. Follow Wilmington Apple on Twitter. Follow Wilmington Apple on Instagram. Subscribe to Wilmington Apple’s daily email newsletter HERE. Got a comment, question, photo, press release, or news tip? Email firstname.lastname@example.org.Share this:TwitterFacebookLike this:Like Loading… Related2 Children Baptized At The Parish Of The TransfigurationIn “Community”2 Children Baptized At The Parish Of The TransfigurationIn “Community”5 Children Baptized At The Parish Of The TransfigurationIn “Community”
[Representational image] Creative CommonsIn a horrifying incident, a 62-year-old man sedated and raped his teenaged daughter in Mumbai for a few months. The accused has been arrested.According to the complaint filed by the victim, the father used to mix sedatives in her dinner every night. When she fell unconscious, he would proceed to rape her, reports Times of India.The man works at a private firm and divorced the victim’s mother five years ago. The victim and her younger siblings were staying with their father.The girl fell something amiss since she used to wake up every morning feeling ill and nauseous. She then suspected that her food had something to do with it. Her suspicions increased when her father insisted that she go to her room immediately after dinner every night.However, on the night of March 27, Wednesday, on the pretext that she is unwell, the girl opted to skip dinner. However, her father brought dinner to her room and insisted that she eat. When he left the room, she threw the food away and pretended that she was asleep. A little while later, her father came into the room and raped her. She tried to fight him off and raise an alarm but her father gagged her and threatened her with dire consequences. The girl’s stepmother and siblings were in the next room.The victim, unable to remain silent on the issue, posted her situation on social media and told her friend. The friend then took her to an NGO, who helped her lodge a complaint against her father with the Naya Nagar police in Mumbai.”We arrested him under section 376 (rape) of the Indian Penal Code (IPC). The Thane court remanded him in police custody till April 1,” Kailash Barve, a senior police inspector at the Naya Nagar police station told Hindustan Times.Other IncidentsUnfortunately, another similar took place in Odisha when a 45-year-old man was arrested for raping his 13-year-old daughter earlier this month. The girl, a student of class eight filed a complaint against her father. The police acted on the complaint and arrested the perpetrator. He was charged under many sections of the Indian Penal Code as well as the Protection of Children from Sexual Offences (POCSO) Act.The father was allegedly raping the little girl repeatedly for a month before she had enough and filed a complaint, reports NDTV.
00:00 /07:44 To embed this piece of audio in your site, please use this code: Listen X We’re learning more about the Trump administration’s tentative trade deal with Mexico to update NAFTA. The handshake agreement is the result of weeks of negotiation between the U.S. and Mexico – without Canada – to bring NAFTA into the 21st century.Tony Payan from Rice University tells News 88. 7 from what we know of the deal so far, it’s a mixed bag.“All in all, is it a gain of certainty in the markets? Yes. Is it an enormous gain? I doubt it,” he said. “There’s still much, much work still pending, and, of course, Canada’s own place at the table is still to be defined.”The deal is essentially a series of bullet points right now – with the exact language yet to be ironed out. But some highlights include changes to vehicle manufacturing tariffs.Right now, vehicles produced in North America can be sold in the U.S. duty-free – as long as about two-thirds of their parts come from North America. But the new deal would increase that to 75 percent. And it would also require a large portion of such vehicles be produced in higher-wage factories.President Trump had also wanted a sunset clause that ended the new agreement after five years – unless it was renewed. However, the new agreement would have a 16-year term, with an option to review and change it at the six-year mark.So, what could this new agreement mean for businesses in Houston and Texas? In the audio above, we have some reaction and analysis from Vance Ginn, senior economist for the Austin-based conservative think tank the Texas Public Policy Foundation, and Chris Tomlinson, business columnist for the Houston Chronicle. Evan Vucci/AP PhotoOutgoing Mexican President Enrique Peña Nieto meets with U.S. President Donald Trump. Share
One way that thermophoresis inside living cells can be used is to measure the binding affinities of molecules. As the scientists explain, the binding of a fluorescently marked molecule such as DNA or a protein leads to a change in the thermophoretic depletion strength. Binding affinities can reveal more detailed information about the interactions of these molecules.”The dream would be to record binding affinities in living cells, i.e., translating the award-winning microscale thermophoresis (MST) technique of our startup company Nanotemper into living cells,” Braun said. “However, the measurement protocol is not yet robust against the shape of the cell, so some more tricks to make it work will be necessary. But we are optimistic—experimental tricks are our specialty.” More information: Maren R. Reichl and Dieter Braun. “Thermophoretic Manipulation of Molecules inside Living Cells.” Journal of the American Chemical Society. DOI: 10.1021/ja506169b Now in a new paper, researchers have demonstrated for the first time that thermophoresis—the movement of molecules due to a temperature gradient rather than an electric field—can be used to measure the movement of DNA and other molecules inside living cells. The paper, by Maren R. Reichl and Dieter Braun at the Ludwig Maximilian University of Munich, is published in a recent issue of The Journal of the American Chemical Society.”Our work shows that the measurement of thermophoresis in living cells is possible—moreover, in parallel across the cell and not at one single point,” Braun told Phys.org.In the new technique, a temperature gradient is applied across a cell by an infrared laser. Fluorescently marked molecules inside the cell move along this temperature gradient from hotter to colder regions. A camera can record this thermophoretic movement, with every camera pixel measuring thermophoresis simultaneously and independently. The technique can be performed in the natural environment of cells in vivo.The researchers demonstrated the use of thermophoresis measurements of DNA in the cytoplasm of living cells. Interestingly, the results revealed that DNA movement in the cytoplasm is slowed down, probably due to molecular crowding. In addition to measuring the movement of DNA, the thermophoresis technique could also measure the movement of proteins, pharmaceutical components, and other molecules in cells as long as they can move through the cytoplasm. Ribosomes, for example, are so large and bound to the endoplasmic reticulum that they cannot easily diffuse through the cytoplasm, making them poor candidates for thermophoresis. Journal information: Journal of the American Chemical Society Thermophoresis measurements of DNA and the dye molecule BCECF in the cytoplasm of living cells. Credit: Reichl and Braun. ©2014 American Chemical Society © 2014 Phys.org Researchers model how migration of DNA molecules is affected by charge, salt species, and salt concentration Citation: Scientists manipulate molecules inside living cells with temperature gradients (2014, September 30) retrieved 18 August 2019 from https://phys.org/news/2014-09-scientists-molecules-cells-temperature-gradients.html (Phys.org) —The ability to make measurements of the biomolecular interactions that occur inside living cells is essential for understanding complex biological processes. But probing the inside of living cells without damaging them is a challenge. The cell membrane shields electrical fields, prohibiting the use of electrophoresis, a technique that is commonly used to analyze biological samples in a variety of areas outside living cells. Set-up of the thermophoresis technique. Heating is provided from below by an IR laser. The molecules move along the temperature gradient, indicated by arrows. Credit: Reichl and Braun. ©2014 American Chemical Society Explore further This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
Though, Union Health Minister JP Nadda has hailed the budget as balanced, progressive, pragmatic and pro-people, various stakeholders have raised their reservations over the budgetary allocation for the heath care sector. Talking to Millennium Post, JNU Professor Ritu Priya said: “The budget is not in the best interest of poor. The government had promised to provide at least 50 life saving drugs for free, which cannot be accomplished with this small amount of the Budget.” Also Read – Need to understand why law graduate’s natural choice is not legal profession: CJI“The Health Ministry had demanded a minimum Rs 50,000 crore from the Finance Ministry to expedite primary health care treatment plans for the poor. Now, how the Health Minister will fulfill those promises?” asked the NJU professor, who is associated with Centre for Social Medicine and Community Health (CSMCH) department. It’s worth mentioning that the Health Ministry and CSMCH work in tandem over policy formulations for public health care.Notably, the present allocation is Rs 2011 crore lesser than the last year’s final outlay which was Rs 35,163 crore after cutting down from the total Rs 39,237 crore announced in the Budget 2014-15. “I have allocated Rs 33,152 crore to the health sector. I urge states to utilise their enhanced resources effectively in these areas,” Jaitley said while presenting the Union Budget in the Lok Sabha.As an initiative to boost the public health sector in the country, the government has also decided to set up six new AIIMS-like institutes in Jammu and Kashmir, Punjab, Tamil Nadu, Himachal Pradesh, Assam and Bihar.
at800, the body set up to test for interference from 4G networks on digital-terrestrial broadcasting in the UK, has appointed Nokia Siemens Networks’ former head of global head of press relations, Ben Roome, as its new CEO.Roome will succeed current boss Simon Beresford-Wylie effective of November 30, 2013. He has been a member of at800’s management team since the company’s launch in March this year, and is being promoted from his current role of director of marketing and communications.
A FRIGHTFULLY GOOD TIME FOR EVERYONE THIS HALLOWE’EN AT NERVE CENTRETHE NERVE CENTRE ShareTweet NERVE Centre joins Derry’s world famous Halloween celebrations with a film programme packed with frights for all ages.On Monday, October 31 and Tuesday, November 1, little monsters and goblins (and larger ones too) are invited along to Nerve Centre’s cinema to enjoy one of its family-friendly films in the new Beanbag Cinema.Families have the choice between the Disney classic Hocus Pocus, the stop-motion animations ParaNorman andCorpse Bride or Roald Dahl’s The Witches. Each screening is followed by exciting and engaging digital workshops aimed at introducing children to filmmaking. Spaces are limited so booking early is advised.In 1999, The Blair Witch Project shocked and confused cinema-goers, many who believed its ‘found footage’ was real. It has been described as ‘teeth grindingly scary… one of the best horrors in recent years’.Tickets for all films and workshops are available from the Nerve Centre box office or www.nervecentre.org.Prepare to be SPOOKED!A FRIGHTFULLY GOOD TIME FOR EVERYONE THIS HALLOWE’EN AT NERVE CENTRE was last modified: October 18th, 2016 by John2John2 Tags: Seventeen years later, the Nerve Centre will host a special screening of the renowned psychological horror film on Friday, October 28 for a whole new generation of teenagers in the Beanbag Cinema.The adult programme includes horrors such as The Boy (Monday 31 October).When Greta takes a job as a nanny she soon discovers that her young charge is a life-sized doll that they family treat like a real boy as a way of coping with the death of their child 20 years earlier.In When the Lights Went Out (Wednesday 26 October) a young family discovers that their new house is inhabited by a violent poltergeist known as ‘The Black Monk’, while The Forest (Tuesday 25 October) starring Natalie Dormer is set in the legendary Aokigahara Forest, a real-life place in Japan where people go to end their lives.