Listen Share 00:00 /01:27 Andrew SchneiderPatrons of Axelrad Beer Garden watch the second 2016 presidential debate, projected on the side of neighboring Luigi’s Pizzeria.It’s been a rough weekend for Donald Trump. Friday’s revelations of a tape, in which the presidential candidate boasted his fame meant he could force himself on women with impunity, cast a long shadow over Sunday’s second presidential debate.That was evident from the mood of the crowd at a watch party at Midtown’s Axelrad Beer Garden. The audience at Axelrad largely favored Hillary Clinton to begin with. Trump’s response, when asked about the tape, didn’t win him any converts.Houston Public Media’s Coverage of Election 2016“I think it’s insulting to men to say that men in general are openly advocating sexual assault, as he is implying by saying that it’s just locker room banter to say those kinds of things,” says Clinton supporter Kris Kory. “And I think it’s insulting to women that he keeps glossing over it and saying that we’re just interpreting it the wrong way or he’s sorry if we’re offended. It’s an offensive and a disgusting thing that he’s said.”There were some Trump supporters in the audience. Zeljko Stajnovic says the Republican candidate defended himself well.“In my honest opinion,” Stajnovic says, “touching upon a locker room conversation that all men have had between each other – when he said it as a private citizen, nonetheless, who had no political aspirations at the time – trying to play that off politically is a low blow.”Tamara Sell, a volunteer deputy voter registrar, had a table set up at the front of the beer garden. She says the debate helped get people involved. “One young lady, she was Hispanic, came earlier and she said, ‘I’m not really sure if want to register to vote.’ And then after hearing the debate, she came back and she’s like, ‘I have to register.’”Sell says she registered more than 70 new voters over the course of the debate. X To embed this piece of audio in your site, please use this code:
One way that thermophoresis inside living cells can be used is to measure the binding affinities of molecules. As the scientists explain, the binding of a fluorescently marked molecule such as DNA or a protein leads to a change in the thermophoretic depletion strength. Binding affinities can reveal more detailed information about the interactions of these molecules.”The dream would be to record binding affinities in living cells, i.e., translating the award-winning microscale thermophoresis (MST) technique of our startup company Nanotemper into living cells,” Braun said. “However, the measurement protocol is not yet robust against the shape of the cell, so some more tricks to make it work will be necessary. But we are optimistic—experimental tricks are our specialty.” More information: Maren R. Reichl and Dieter Braun. “Thermophoretic Manipulation of Molecules inside Living Cells.” Journal of the American Chemical Society. DOI: 10.1021/ja506169b Now in a new paper, researchers have demonstrated for the first time that thermophoresis—the movement of molecules due to a temperature gradient rather than an electric field—can be used to measure the movement of DNA and other molecules inside living cells. The paper, by Maren R. Reichl and Dieter Braun at the Ludwig Maximilian University of Munich, is published in a recent issue of The Journal of the American Chemical Society.”Our work shows that the measurement of thermophoresis in living cells is possible—moreover, in parallel across the cell and not at one single point,” Braun told Phys.org.In the new technique, a temperature gradient is applied across a cell by an infrared laser. Fluorescently marked molecules inside the cell move along this temperature gradient from hotter to colder regions. A camera can record this thermophoretic movement, with every camera pixel measuring thermophoresis simultaneously and independently. The technique can be performed in the natural environment of cells in vivo.The researchers demonstrated the use of thermophoresis measurements of DNA in the cytoplasm of living cells. Interestingly, the results revealed that DNA movement in the cytoplasm is slowed down, probably due to molecular crowding. In addition to measuring the movement of DNA, the thermophoresis technique could also measure the movement of proteins, pharmaceutical components, and other molecules in cells as long as they can move through the cytoplasm. Ribosomes, for example, are so large and bound to the endoplasmic reticulum that they cannot easily diffuse through the cytoplasm, making them poor candidates for thermophoresis. Journal information: Journal of the American Chemical Society Thermophoresis measurements of DNA and the dye molecule BCECF in the cytoplasm of living cells. Credit: Reichl and Braun. ©2014 American Chemical Society © 2014 Phys.org Researchers model how migration of DNA molecules is affected by charge, salt species, and salt concentration Citation: Scientists manipulate molecules inside living cells with temperature gradients (2014, September 30) retrieved 18 August 2019 from https://phys.org/news/2014-09-scientists-molecules-cells-temperature-gradients.html (Phys.org) —The ability to make measurements of the biomolecular interactions that occur inside living cells is essential for understanding complex biological processes. But probing the inside of living cells without damaging them is a challenge. The cell membrane shields electrical fields, prohibiting the use of electrophoresis, a technique that is commonly used to analyze biological samples in a variety of areas outside living cells. Set-up of the thermophoresis technique. Heating is provided from below by an IR laser. The molecules move along the temperature gradient, indicated by arrows. Credit: Reichl and Braun. ©2014 American Chemical Society Explore further This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
In a move signalling its expansion into the Australian and New Zealand market, Hertz today announced the acquisition of budget car rental company Ace Rental Cars and an alliance with Apollo Motorhome Holidays.At a press conference in Sydney, visiting Hertz International president Michel Taride said the two ventures were steps in Hertz’s plans to diversify the company’s brand and product offerings.Despite continuing with its core business, Mr Taride said, “we recognise also that we need to continue refining and developing our product offerings in order to meet the changing requirements of our customers, and to position ourselves for emerging opportunities.”Mr Taride said he was surprised to learn that almost half of the car rental market in New Zealand was in the “second tier” segment and that the aim of the Ace acquisition was to “try to grow the brand further” in Australia and across the Tasman. “Even in a downturn, people still want to travel and we want to be part of the low cost offerings without diluting the product.”“Companies with a long history like ours have to adapt.”Seeing the growing popularity of campervan and motorhome holidays, Hertz has partnered with the largest privately-owned operator of recreational vehicles in the world, Apollo Motorhome Holidays.According to Mr Taride, the commercial alliance with Apollo will allow Hertz to enter the Australian motorhome market quickly with a ready-made product range. Effective 1 April 2011, motorhome customers will be able to book Apollo vehicles through Hertz websites in Australia and New Zealand or via the Hertz telephone reservations network.Hertz recently acquired Melbourne-based car sharing company Flexicar and partnered with Australia’s Virgin Blue Airlines group and Middle Eastern Etihad. “[These acquisitions and partnerships] are all strategic decisions designed to ensure that we have the travel products and relationships that our customers expect in various market segments,” Mr Taride said.Hertz estimates the value of the Australian rental car market at AUD1.5 billion, a large portion of which Hertz shares in. Michel and Karina Source = e-Travel Blackboard: M.H